EVENT DETAILS AND ABSTRACT

Title: Micromechanical study of proteins interacting with single DNA molecules
Speaker: John F. Marko
Speaker Info: University of Illinois at Chicago
Brief Description:
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Abstract:

I will discuss the use of micromechanical assays - essentially measurements of elasticity - as methods to study how DNA is organized, ultimately into chromosomes. I will discuss studies at three scales of complexity. First, results of single-DNA micromanipulation experiments to study proteins which compact DNA by bending it will be presented. Next, I will show how similar techniques can be applied to the study of the dynamics of assembly of chromatin fiber onto a single DNA molecule. Finally I will describe experiments which probe the internal organization of entire mitotic chromosomes isolated from dividing cells.